Is an observed non-co-linear RNA product spliced intrans, incisor justin vitro?
نویسندگان
چکیده
منابع مشابه
Is an observed non-co-linear RNA product spliced in trans, in cis or just in vitro?
Global transcriptome investigations often result in the detection of an enormous number of transcripts composed of non-co-linear sequence fragments. Such 'aberrant' transcript products may arise from post-transcriptional events or genetic rearrangements, or may otherwise be false positives (sequencing/alignment errors or in vitro artifacts). Moreover, post-transcriptionally non-co-linear ('PtNc...
متن کاملTranslation of globin RNA spliced in vitro.
Pre-mRNA (precursor mRNA) enriched for globin-gene transcription products was prepared from murine erythroleukaemia cells induced to differentiate with dimethyl sulphoxide. The pre-mRNA was prepared from nuclear RNA by oligo(dT)-cellulose chromatography followed by sucrose-gradient centrifugation. The pre-mRNA thus obtained was utilized as the substrate for a splicing assay based on sequential ...
متن کاملSupersplat—spliced RNA-seq alignment
MOTIVATION High-throughput sequencing technologies have recently made deep interrogation of expressed transcript sequences practical, both economically and temporally. Identification of intron/exon boundaries is an essential part of genome annotation, yet remains a challenge. Here, we present supersplat, a method for unbiased splice-junction discovery through empirical RNA-seq data. RESULTS U...
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چکیده ندارد.
15 صفحه اولTrypanosome spliced-leader-associated RNA (SLA1) localization and implications for spliced-leader RNA biogenesis.
Spliced-leader-associated RNA (SLA1) guides the pseudouridylation at position -12 (relative to the 5' splice site) of the spliced-leader (SL) RNA in all trypanosomatid species. Nevertheless, the exact role of this RNA is currently unknown. Here, we demonstrate that the absence of pseudouridine on Leptomonas collosoma SL RNA has only a minor effect on the ability of this RNA to function in trans...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2014
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gku643